Molecular Biology Lab
The Reproductive Physiology Group performs their molecular biology research in laboratory spaces located in Hultz Hall 110 and 119c. Located in this lab is equipment required for tissue homogenization, nucleic acid and protein purification, RNA quantification and integrity analysis, cDNA synthesis, PCR, and qPCR. Nuclease-free work spaces are maintained for molecular work in these labs.
Specific pieces of equipment located in the Molecular Biology lab are listed below and organized by required use:
General Equipment
Milli-Q Advantage A10 Ultrapure Water Purification System
Tissue Homogenization
Kinematica Polytron Homogenizer (large and small generators)
TissueLyser LT with 12 tube adapter
Nucleic Acid and Protein Purification
Two Eppendorf 5430 centrifuges (with rotors to accommodate 30 microcentrifuge tubes and 96 well plates)
RNA Analysis
Access to a Nanodrop 2000c Spectrophotometer
PCR and qPCR
MJ Research Thermal Cycler
ABI 7000 Sequence Detection System
