Oakes Irrigation Research Site
Carrington Research Extension Center * North Dakota State University
P.O. Box 531, Oakes, ND 58474-0531, Phone: (701) 742-2744, FAX: (701) 742-2700,
E-mail: Kelly.c.Cooper@ndsu.edu
Optimizing Fungicide Application Timing for Sclerotinia Control in Soybeans
M. Wunsch, M. Schaefer, B. Kraft, S. Kallis, K. Cooper, L. Besemann, H. Eslinger 
METHODS
General Agronomics: The study was on a Maddock sandy loam soil type. The previous crop was spring wheat and the tillage operation consisted of field cultivation May 2.  The maintenance herbicide applications were:  PRE-EMERGENCE Spartan Elite (sulfentrazone, 0.7 lb ai/gal, and s-metolachlor, 6.3 lb ai/gal; FMC Corp., Philadelphia, PA) was applied at 22 fl oz/acre on May 19 and  POST‑EMERGENCE:  Roundup PowerMax (5.5 lbs/gal of the a.i. glyphosate = 4.5 lbs/gal of the acid equivalent; Monsanto Corp., St. Louis, MO) was applied June 13 at 32 fl oz/acre with 1 lb AMS/10 gal and 0.125% v/v NIS.
Experimental design: A completely randomized block design with six replicates, with fungicide timing evaluated within each row spacing as a separate experiment.  The seeded plot size was 5 feet (center to center) by 25 feet long. The harvested plot size was 5 feet (center to center) and approximately 22 feet long. Every treatment plot was separated by a non-harvested buffer plot.  
Planting details: Pioneer 'P07T50R' (0.7 maturity) was seeded at 165,000 pure live seeds/acre. The study was planted on May 17, 2016.  Seed treatments used were Apron Maxx RTA (5.0 fl oz/100 lbs seed) + Imidacloprid 4ST (3 fl oz/100 lbs seed).  Imidacloprid 4ST (Willowood) contains 4 lbs/gal thiamethoxam; Apron Maxx RTA (Syngenta) contains 0.096 lb/gal mefenoxam and 0.064 lb/gal fludioxonil.  Rhizobium inoculant:  BYSI-N granular inoculant for soybeans (100 million Bradyrhizobium japonicum per gram; Lallemand Specialties Canada, Saskatoon, SK) was applied in-furrow with the seed at a rate of 10.3 lbs/acre.
Fungicide applications:  
Application details: Fungicides were applied with a 60-inch hand boom equipped with 4 equally spaced TeeJet 80015VS flat-fan nozzles at a spray volume of 15 gal water/A operated at 40 psi.
Application A: 07/08/2016; 47% of plants at the R1 growth stage (at least one open blossom); 90-100% canopy closure in the 14-inch rows and 50% canopy closure in the 28-inch rows.  Wind speed - 3 MPH, Temperature 79°F, R Humidity 53%.
Application B: 07/15/2016; 100% of plants at the R2 growth stage (an open blossom at one of the top two nodes; could not spray earlier because the wind speed was too high.); 100% canopy closure in the narrow (14-inch) rows and 75-90% canopy closure in the wide (28-inch rows); Wind speed - 2 MPH,   Temperature 75°F, R Humidity 48%.
Application C: 07/18/2016; 100% of plants at the R2 growth stage; 100% canopy closure in the narrow (14-inch) rows and 90-100% canopy closure in the wide (28-inch) rows. Wind speed - 5 MPH, Temperature 76°F, R Humidity 69% R2-100%.
Application D:  07/20/2016; 90-100% of plants at the R3 growth stage (a pod at least 3/16" long at one of the top two nodes); 100% canopy closure in the narrow (14-inch) rows and 95-100% canopy closure in the wide (28-inch) rows.  Wind speed - 5 MPH, Temperature 80°F, R Humidity 87%.
Notes and disease establishment: The trial was established on a site with a previous history of Sclerotinia epidemics. To sustain the soil moisture necessary for apothecia development, the trial was irrigated aggressively to create conditions favorable for apothecia production and Sclerotinia infection.  Sclerotinia incidence and severity were assessed on September 14 at the late R7 growth stage using the 0 to 3 scale developed by Craig Grau (Grau and Radke 1984; Plant Disease 68: 56-58):  0 = no symptoms, 1 = lesions on lateral branches only, 2 = lesions on main stem, no wilt, and normal pod development, 3 = lesions on main stem resulting in wilting, poor pod fill, and plant death.  All plants were assessed in the middle two rows of four-row plots (14-inch row spacing) and in one of two rows of two-row plots (28-inch row spacing).
Harvest, seed yield and quality assessment: The trial was harvested on September 26. To facilitate accurate yield assessment, plot lengths were measured shortly before harvest. Yields were calculated on the basis of a 5-ft plot width and the measured plot length.  Seed moisture was assessed after the grain was cleaned. Seed yield and quality results were adjusted from the grain actual moisture to a standard 13% moisture level.
FUNDED BY THE NORTH DAKOTA SOYBEAN COUNCIL
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