Polymerase Chain Reaction and DNA Sequencing for Detection of Ovine Herpesvirus 2
in American Bison (Bison bison)

Elizabeth A. Sauske and Neil W. Dyer


From J. Vet Diagn. Invest.14:40-46 (2002)


erum samples were collected at slaughter from 226 24-30-month-old ranch-raised, clinically normal American bison (Bison bison) bulls from North Dakota, Minnesota, Kansas, and Manitoba to assess the presence of antibodies to ovine herpesvirus 2 (OHV-2).  Antibodies to OHV-2 were detected by competitive inhibition enzyme-linked immunosorbent assay in 10 of 226 (4.40%) samples.  Polymerase chain reaction (PCR) analysis of sera positive for OHV-2 DNA demonstrated a 238 kilobase fragment.  The nucleotide sequence of the PCR-positive samples in comparison to the reported OHV-2 nucleotide sequence resulted in a homology range of 82.8 to 95.4 percent.


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