Mary Lynn Johnson
Adjunct Professor/Research Associate
Office: 267 Hultz Hall
Phone: (701) 231-6747
E-mail: mary.lynn.johnson@ndsu.edu
Research interests:
Research interests center on control of cell and blood vessel growth and cell-cell communication in reproductive tissues and healing wounds. Expertise is in the application of molecular techniques to answer research questions. Real time RT-PCR for assay of mRNA expression and SNP analyses for genotyping sheep for resistance to scrapies.
Training/Education:
Postdoctoral fellowship , Reproductive Physiology, Animal and Range Sciences, NDSU -1996-1998
Ph.D., Program, Cellular and Molecular Biology; Animal and Range Sciences, NDSU -1989-1995
M.S., Bacteriology, NDSU - 1984-1986
B.S., Medical Technology, University of North Dakota, Grand Forks, ND -1960-1964
Teaching Experience:
Fixed-term of two years, Departments of Biology and Chemistry, Moorhead State University, Moorhead, MN. Taught a Molecular Biology lecture and laboratory, Chemistry 102 lecture and biochemistry, chemistry and biology labs. 1998-2000.
Selected Publications:
Johnson, M.L. , L.P. Reynolds, and D.A. Redmer. Quantification of lane-to-lane loading of poly(A) RNA using a biotinylated oligo(dT) probe and chemiluminescent detection. BioTechniques 19:712-715. 1995
Zheng J, M.L. Johnson , D.A. Redmer, L.P. Reynolds. Estrogen and P4 receptors, cell proliferation, and c-fos expression in the ovine uterus during early pregnancy. Endocrinology 137:340-348. 1996.
Johnson, M.L. , S.D. Killilea, D.A. Redmer, and L.P. Reynolds. Production of heparin-binding endothelial mitogens by bovine uterine fibroblastic and epithelial cells. Can. J. Physiol. Pharmacol. 74:1079-1086. 1996.
Johnson, M.L. , D.A. Redmer, and L.P. Reynolds. Uterine growth, cell proliferation, and c- fos proto-oncogene expression throughout the estrous cycle in ewes. Biol. Reprod. 56:393-401. 1997.
Johnson, M.L. , L.P. Reynolds, and D.A. Redmer. Effects of ovarian steroids on uterine growth, morphology, and cell proliferation in ovariectomized, steroid-treated ewes. Biol. Reprod. 57:588-596. 1997.
Johnson, M.L. , D.A. Redmer, L.P. Reynolds, and A.T. Grazul-Bilska. Cloning and evaluation of expression of connexin (Cx)43, Cx32 and Cx26 mRNA in sheep tissues. 2001 International Gap Junction Conference, Honolulu , Hawaii , August 4-9, 2001 .
Johnson, M.L. , L.P. Reynolds, D.A. Redmer, J.J. Bilski, and A.T. Grazul-Bilska. Gap junctional intercellular communication of bovine granulosa and thecal cells from antral follicles: Effects of luteinizing hormone and follicle stimulating hormone. Endocrine 18:261-270, 2002.
Grazul-Bilska, A.T., M.L. Johnson, J.J. Bilski, D.A. Redmer, L.P. Reynolds, A. Abdullah, and K.M. Abdullah. Wound healing: The role of growth factors. Drugs of Today. 2003. Invited review.
GenBank Submissions: Johnson, M.L. , Redmer, D.A., Reynolds, L.P. and Grazul-Bilska, A.T.
Ovis aries mRNA for connexin43, gap junctional protein alpha 1, partial cds . Accession No. AY074716.
Ovis aries mRNA for connexin26, gap junctional protein beta 2 , partial cds. Accession No. AY074717.